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Title   인체 유암조직에서 DNA Ploidy , S - phase Activity 와 c-erbB-2 유전자 단백발현이 예후에 미치는 영향 ( DNA Ploidy , S - phase Activity and c-erbB-2 Oncogene Protein Expression in Breast Cancer and its relationship to Prognosis )
Publicationinfo   1992 Jan; 024(01): 73-82.
Key_word   Breast cancer, Ploidy, S-phase fraction, c-erbB-2 oncogene
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Abstract   In breast carcinomas, identification of poor prognostic factors is utmost important not only be- cause these factors play key roles in deciding adjuvant therapy but also certain factors are thought to discriminate high risk group in the same stage. Axillary lymph node status is the single most important prognostic fator for patients with breast cancer. Nevertheless 25 to 30% of node negative patients do recur and die from their disease within 10 years. Tumor size or hormone receptor status may play risk factor and the latter is important to decide hormonal treatment. The technique of flow cytometry offers a possibility to examine the DNA content and cell cycle, the measurement of DNA ploidy and S-phase fraction in breast carcinoma has been tried in many institutes. The development of molecular biology gave insight into oncogene and some rePorted overexpression of erbB-2 oncogene in breast carcinomas. So, in this study though not enough cases, we tried to identify the risk factors in the breast carci- nomas whether these factors can tell the risk. Using paraffin-embedded tumor blocks of 86 breast carcinomas, DNA plody and S-phase fraction were measured by flow cytometric analysis and erbB- 2 oncogene protein expression was detected by immunocytochemical method. There were 69% of less than age 50 and 49% of them were aneuploid and 31% of these patients were over 50 and 37% of them were aneuploid. Here we have more aneuploidy in less than age 50 group but there is no sta- tistical significance. The tumor size was mostly less than 5cm and in the patients over than 5cm group there were 83% of aneuploidy with statistical significance. But there were no difference of ploidy according to node status or tumor grade. There were sig- nificantly larger number of aneuploidy in erbB-2 negative group when compared to erbB-2 positive group. We had median value of S-phase at 20% and when we evaluate the correlation between S-phase and other pragnostic variables there were significantly large number of higher S-phase in the tumor size over than 4 cm, but the other factars didnt have any significant relation with S-phase. erbB-2 expression significantly related with ploidy but there were no signifcant relations between other prognostic factors. The results of disease free survival curves according to individual prognostic factors can be summarized as lymph node status plays the most independent proanostic factor, but we couldn't find any significant role of DNA ploidy or S-phase fraction as an independent prognostic factor. erbB-2 oncogene expression possibly plays as a significant prognostic factor and more prognostic if combined with node status in the relatively early stage breast carcinomas. Though not conclusive, we can suggest that DNA and S-phase fraction as well as erbB-2 oncogene expression would be expected to play certain role to prognosticate breast carcinomas.
저 자   노동영(Dong Young Noh),최국진(Kuk Jin Choe),김진복(Jin Pok Kim),박인애(In Ae Park),박성회(Seong Hoe Park),유근영(Byung Joo Park)